Wireless ad hoc communication and mobile networking/computing have applications in a variety of environments, such as conferences, hospitals, battlefields, and disaster-recovery/rescue operations, and are also being actively investigated as an alternative paradigm for Internet connectivity in both urban and rural areas. Wireless sensor and actuator networks are being deployed for enhancing industrial control processes and supply-chains, and for various forms of environmental monitoring. IEEE MASS 2016 aims at addressing research advances in (i) algorithms and theory, (ii) systems, protocol, and applications, and (iii) experimental evaluation and testbeds, in multi-hop wireless ad hoc and sensor networks, covering topics ranging from technology issues to applications and test-bed development.