The 5th international conference on Novel Enzymes (INEC16) aims to provide a forum for the presentation of the most exciting advances and new findings concerning enzymes. The conference is a continuation of the conference series on Novel Enzymes, of which the previous was held in 2014 in Ghent, Belgium. The goal of the current conference is to provide an overview on recent developments and future perspectives on enzymology research. Emphasis will be given to discovery of new enzymes, engineering approaches (including novel assays), and newly developed enzyme applications. The conference is aimed at bringing together researchers, from academia and industry, working in the field of enzymology, and to facilitate stimulating discussions. Keynote lectures are delivered by reputed academic and industrial scientists who will present new developments in diverse areas of molecular and applied enzymology.