We are pleased to announce a fourth meeting on Single Cell Analyses, which will be held at Cold Spring Harbor Laboratory, New York. The meeting will begin with dinner and an an evening session on Wednesday November 11, and conclude with lunch on Saturday, November 14, 2015. The proposed meeting will assemble the leaders in the field, together with junior faculty, postdoctoral fellows and graduate students, to discuss new, cutting-edge developments and techniques in single cell analysis in bacterial, yeast, plant and animal systems.