The zebrafish is a powerful genetic model for almost all aspects of vertebrate development The advantage of using zebrafish os human disease model through various approaches such os forward genetics, reverse genetics, chemical genetics, precision medicine and live imaging, has been extensively explored. This Symposium focuses on the latest development in identifying genes controlling diverse developmental processes, unraveling molecular mechanisms of disease-related genes, screening of potential therapeutic drugs and thereof the possible application of these recent achievements in translational and regenerative medicine research.