Biocatalysis encompasses the use of enzymes or whole cell systems for effecting the conversion of readily available, inexpensive starting materials to high value products. Enzymes are fully recyclable catalytic proteins that frequently display exquisite chemo-, enantio- and regioselectivity and operate under mild conditions of pH and temperature. These characteristics make them cost-effective and sustainable catalysts for a wide range of chemical transformations. Modern tools of protein discovery and engineering as well as advances in molecular biology and protein structure aid the development of biocatalysts and their tailor-designed integration into industrial processes. Consequently, they find wide application in the production of pharmaceutical intermediates, novel materials and diagnostics, as well as fine, performance and commodity chemicals. The Biocatalysis Gordon Research Conference highlights the best science, technologies and case studies to provide in-depth understanding of the development and practical use of biocatalysts around the world.