The Sixth International Workshop on Bio-Design Automation (IWBDA) will bring together researchers from the synthetic biology, systems biology, and design automation communities to discuss concepts, methodologies and software tools for the computational analysis and synthesis of biological systems. The field of synthetic biology, still in its early stages, has largely been driven by experimental expertise, and much of its success can be attributed to the skill of the researchers in specific domains of biology. There has been a concerted effort to assemble repositories of standardized components; however, creating and integrating synthetic components remains an ad hoc process. Inspired by these challenges, the field has seen a proliferation of efforts to create computer-aided design tools addressing synthetic biology's specific design needs, many drawing on prior expertise from the electronic design automation (EDA) community. The IWBDA offers a forum for cross-disciplinary discussion, with the aim of seeding and fostering collaboration between the biological and the design automation research communities. IWBDA is organized by the non-profit Bio-Design Automation Consortium (BDAC). BDAC is an officially recognized 501(c)(3) tax exempt organization.