There is a huge demand for research tools allowing one to “see” nanostructures and to characterize and understand materials as well as biochemical process at nanoscale resolution. Optical techniques such as imaging and spectroscopy at nanoscale make this possible. Optical imaging with spatial resolution far beyond the diffraction limits of light together with spectroscopic studies with highly localized optical fields have pushed the limits of spatial resolution and sensitivity to new scales. Continuous improvements open ways to novel applications at the forefront of scientific knowledge. The purpose of this interdisciplinary conference is to encompass all aspects of nano-imaging and nano-spectroscopy, including theory and novel concepts, experimental demonstration of novel concepts, major developmental progress and applications to any field in science, in particular, biology, medicine, and the material sciences.