The 2013 Gordon Research Conference on Three-Dimensional Electron Microscopy (3DEM) will present leading research in three-dimensional structure determination of biological material, from macromolecules to cells. The meeting will highlight recent progress in the areas of single particle cryo-EM, electron tomography, and electron microscopy of ordered assemblies and 2D crystals. This will be the 19th meeting of a series that began in 1985. The progress in the field has been remarkable, and 3DEM has become a key method in structural biology and cell biology. The technique is unique in allowing determination of structure and conformational dynamics of a wide variety of specimens ranging from macromolecules and macromolecular assemblies to viruses, organelles and whole cells in their native environment. A focus at the conference will be on technological advances in instrumentation, software development, data processing, and tools for interpretation and validation that lead to improvements in quality, resolution, and reliability of the EM maps. Ample time will be given to demonstrating how 3DEM studies help elucidate biological function. The Conference will bring together world-leading experts in the field, junior researchers and also representatives of industrial partners. Following the tradition of previous conferences the program will offer an attractive combination of lectures and poster sessions. There will be two sessions with poster presenters selected for short talks. The program will also provide excellent opportunities for discussions and informal gatherings. Due to limited attendance (~150 participants) and the over-subscribed nature of previous meetings, early registration is encouraged.