Optofluidics merge optics and fluidics from the micro- to the nanoscale and enable new functionalities. Emerging fields of optofluidic research and applications may be found in the sector of sustainability energy, advanced chip-based sensor technology, and biophotonic handling and analysis in fluid media. The 1st EOS Conference on Optofluidics was a great success, with participation from leaders in the optofluidics community from around the world. This 2nd EOS conference will build on that momentum, exploring the latest developments in the field of optofluidics, and connecting experts from academia, start-ups and established companies.