The 7th IEEE International Conference on Systems Biology (ISB 2013), organized by Chinese Academy of Sciences and Hunan University will be held in Huangshan (Yellow Mountain), China, August 22-25, 2013. The conference is sponsored by National Natural Science Foundation of China (NSFC), Academy of Mathematics and Systems Sciences of CAS (AMSS), Shanghai Institutes for Biological Sciences of CAS (SIBS), Hunan University, Computational Systems Biology Society of ORSC, Systems Biology Technical Committee of IEEE SMC Society, and also sponsored by IET. Systems Biology and Bioinformatics have become intensive research topics in the recent past decade and attracted great many leading scientists working in Biology, Physics, Mathematics and Computer Science. Optimization, Statistics, and many other mathematical methods have been widely used in the field. Following the successful ISB conferences series from 2007, the purpose of ISB 2013 is to extend the international forum for scientists, researchers, educators, and practitioners to exchange ideas and approaches, to present research findings and state-of-the-art solutions in this interdisciplinary field, including mathematical methods and its applications in biosciences and researches on various aspects of Systems Biology, such as integration of genome-wide microarray, proteomic, and metabolomic data, inference and comparison of biological networks, and model testing through design of experiments.