583 / 2018-11-12 08:37:45

Effects of three microbial inoculation on mixed storage quality and microbial community of dried corn straw and cabbage waste

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The objective of this study was to examine the effect of three microbial inoculants (Lactobacillus Plantarum, Lactobacillus buchneri and Pichia anomala) on mixed-storage quality for dried corn straw and cabbage waste and microbial community diversity. Four treatments were applied: (1) mixed silage without any additives (control treatment, ME); (2) 1×106 cfu (colony forming units)/g of Lactobacillus plantarum (LP); (3) 1×106 cfu/g of Lactobacillus buchneri (LB); (4) 1×106 cfu/g of Pichia anomala (PA) during mixed-storage by the theory of ensilage. The results showed that the content of water soluble carbohydrates, dry matter and acetic acid in three microbial treatments increased significantly (P<0.05) compared to the control treatment ME. In contrast, the content of acid detergent fiber, holocellulose and pH value in three microbial treatments decreased significantly (P<0.05) during storage for 60 days, and the ammonia nitrogen-total nitrogen ratio decreased significantly (P<0.05) during storage for 30 days. Comparing to other treatments, the content of lactic acid, lactic acid-acetic acid and lactic acid-total organic acid ratios increased significantly (P<0.05) in PA. Moreover, the mixed-silage quality in PA was markedly higher than the other treatments according to Flieg grading method. The microbial community diversity analysis found that Lactobacillus and Enterobacter were the dominant bacterial in three microbial treatments, and the relative abundance of spoilage bacteria Enterobacter was significantly lower than that in the control treatment ME. The relative abundance of Flavobacterium and Pedobacter in PA was significantly lower than that in other treatments. In summary, the findings of this study suggested that the addition of three microorganism inoculants all could improve storage quality of mixed-silage, and the effectiveness by the addition of Pichia anomala was preferable for the mixed-storage of dried corn straw and cabbage waste.