Abstract: Bio-oils derived from lignocellulosic biomass was considered as one of the promising alternative renewable biofuels to substitute of fossil fuels. How to upgrading the raw bio-oils to biofuels is become one of the obstacle for scale up using of bio-oils. In this paper, aqueous phase hydrodeoxygenation was introduced to upgrading the lignin derived bio-oils and eugenol was chosen as the model compound. Incipient wetness impregnation method, ammonia grafting method, direct synthesis method were used to load nickel onto Al-SBA-15. The catalytic activity of eugenol hydrodeoxygenation was investigated over different nickel based Al-SBA-15 catalysts. The physicochemical characterization was tested by XRD, SEM, TEM, FT-IR and N2 sorption. It showed that nickel silicate and NiO are formed on the surface of nickel based Al-SBA-15 prepared by ammonia grafting method. While, NiO was existed on the surface of nickel based Al-SBA-15 obtained by the incipient wetness impregnation and direct synthesis method. Ni/Al-SBA-15 prepared by direct synthesis method has high surface area (792 m2 g-1) and the highest pore volume (0.619 cm3 g-1). Ni/Al-SBA-15 obtained by ammonia grafting method has the lower surface area (304 m2 g-1) and the lower pore volume (0.290 cm3 g-1). Ammonia grafting method can enhance the adsorption of nickel species onto the surface of Al-SBA-15 and the highest nickel content of 32 % was obtained by ammonia grafting method. During the aqueous phase hydrodeoxygenation of eugenol to hydrocarbon, catalysts prepared by ammonia grafting method exhibited the highest catalytic activity.