The osteogenic effect of mesenchymal stem cells with directionality via titanium dioxide nano-rod quartz plate.
Yu Wang 1, Huiming Wang 1, Mengfei Yu 1
1 The Affiliated Stomatologic Hospital of Medical College, Zhejiang University
yu wang @zju.edu.cn
Introduction
A large number of tissue cells in the human body have a certain direction in functional state. Some research achievements have been made in the osteogenesis of hMSCs on titanium dioxide nanostructure . However, the osteogenesis of directional cells has not been thoroughly studied. The purpose of this study was to illustrate the osteogenesis of directed human bone marrow mesenchymal stem cells (hMSCs) cultured on the surface structure of titanium dioxide nanoparticles
Materials and methods
Preparation of titanium dioxide nanorod quartz plate: Titanium dioxide nano-rod quartz plate is made on the surface of 10*10*1mm quartz plate by suspension coating method.
Preparation of light strip on quartz plate surface: The quartz plate is covered with a palisade mask with a spacing of 30um with 365nm wavelength uv lamp illumination for 1h.
Cell culture and seeding on plates: HMSC cells were cultured with low-sugar medium to the third generation, and were inoculated on the surface of quartz plate in 24-hole plate at the concentration of 5W/ hole. After 24h, the quartz plate was transferred to the new 24-hole plate and cultured with low-sugar medium.
Results
The cells were arranged in a fusiform directional arrangement on a strip of titanium dioxide nanorods quartz plate. Cell proliferation experiments showed that the cell growth rate was better than the control group. PCR results of 7 days and 14 days showed that the expression of targeted cultured cells on runx-2, OCN, ALP, BMP2 and other osteogenic genes was significantly different from that of the control group.
Conclusions
This study combines titanium dioxide nanostructures with cell direction to observe cell osteogenesis, and the results showed that titanium dioxide nanorods based on the surface structure of directional hMSC had stronger osteogenesis capacity, providing a new reference for bone tissue regeneration engineering.
References
1.俞梦飞. 光控细胞薄层技术及其在组织工程中的应用[D]. 浙江大学, 2015.
2.Dong L, Cheng K, Weng W, et al. Hydrothermal growth of rutile TiO2 nanorod films on titanium substrates[J]. Thin Solid Films, 2011, 519(15):4634-4640.
3. Liu C, Zhou Y, Sun M, et al. Light-induced cell alignment and harvest for anisotropic cell sheet technology[J]. Acs Appl Mater Interfaces, 2017, 9(42):36513-36524.