93 / 2018-08-02 12:13:11

The Construction of GBM Microenvironment Based on 3D Bioprinting

3D bioprinting,glioblastoma multiforme(GBM),GBM invasion model,tumor model

全体主题 > iv. Design and fabrication of organ-on-a-chip, disease models, bioreactors, and medical and diagnostic devices

As an emerging biofabrication platform enables the creation of high-resolution 3D structures, 3D bioprinting has been extensively used in the past decade to model multiple organs and diseases. More recently, this versatile technique has further found its application in studying cancer genesis, growth, metastasis, and drug responses through the creation of accurate models that reproduce the complexity of the cancer microenvironment. Firstly our research will focus on cancer biology and current tumor models. We then detail the existing bioprinting strategies including the selection of bioinks and bioprinting of vascular structures, etc. After which we will take the current tumor models as reference and target glioblastoma multiforme(GBM), the most aggressive tumor within the brain. Then we will design and print several in vitro GBM model structures with stable mechanical properties and high cells survival rate through multiple experiments, optimize printing process and parameters to provide reasonable experimental model reference for further exploration of the invasion characteristics of GBM. In the end, we will combine solid square structure and multilayer grid structure to build the scaffold of GBM invasion model based on theoretical analyses and preliminary experiments. GBM spheroids will be injected onto the scaffold evenly. The model is expected to simulate in vivo invasion process of GBM to study some of the biological problems, such as the differences of gene expression before and after GBM invasion, and the induction of GBM invasion by glucose concentration gradient, etc. The model also lays a certain theoretical foundation to reveal the molecular mechanism of the invasive growth of GBM, and provides new methods and effective means of studying GBM invasion process. The model can be adapted to other biological systems and used as a valuable tool to model cell-cell interactions and to control microenvironment in other systems.