90 / 2018-06-29 14:05:23

3D Printing of Multi-scale Scaffolds by On/off Electrohydrodynamic Field

3D scaffold,3D printing,Melt electrospinning writing,Multi-scale scaffold,Cell culture

全体主题 > vi. BAMOS (Biomaterials and Additive Manufacturing: Osteochondral Scaffold innovation)

In tissue engineering, three-dimensional(3D) porous structures is critical to facilitate cell attachment, growth and proliferation. Traditional fused deposition modeling (FDM) has a limitation whereby its patterning resolution is too low at around 200μm, therefore, it can’t implement advanced biomedical functions. Recently, melt electrospinning writing (MEW) has received much attention for well controlled micro-scale structures manufacturing at round 10μm, however, it can’t offer enough strength support. In this paper, we combine the two techniques to fabricate multi-scale PCL-based scaffolds, where FDM offer the macro shape and strength support, and MEW offer a favorable environment for cell growth. First, micro-scale pattern was fabricated by MEW, Next, macro-scale pattern was fabricated by FDM. Then, repeat the previous two step. Finally, we can obtain the well controlled 3D cross-scale scaffolds at both macro scale and micro scale. Cell viability, proliferation, and morphology were systematically studied, and the result indicate that the multi-scale scaffolds were favorable for cell attachment and growth.