Bioprinting aims to produce organs which are complex systems with highly ordered structure to sustain functions for the human body. The bioink presents 3D microenvironmental conditions to control cell behaviour. However, current bioinks are limited by simple structure and composition. The decellularized matrix has been tried to amplify bioactivity of bioink. Herein, we modify the conventional protocol and get a novel hydrogel matrix from the discarded human tissues. The matrix could be used as bioink in 3D printing and one tissue derived decellularized extracellular matrix was named dECM-X (Figure 1A). Excitingly, it showed that the printed structures from dECM-X supported stem cell proliferation and in the animals there would be less immune-rejection compared to dECM from some other tissues. Based on the in vivo study of the printed scaffolds, it is demonstrated that tissue injuries such as skin burning/damage can be immediately repaired because of the high bioactivity of vascular network regeneration and tissue remodelling. Furthermore, for the decellularized work, we have generated some dECM patches from the uterus. After implantation, there would be myometrium and endometrium formation to recapitulate native tissue structure (Figure 1B). Importantly, there were also glands and blood vessels organization and the isolated tissues could react to estrogen and progestin. Finally, the host rats could be pregnant and healthy offsprings were generated. The further work will concentrate on the composition analysis. Summarily, the ECM from natural tissues provides many opportunities for bioink development and tissue reestablishment. Our work also promotes the application of ECM biomaterials. Bioprinting aims to produce organs which are complex systems with highly ordered structure to sustain functions for the human body. The bioink presents 3D microenvironmental conditions to control cell behaviour. However, current bioinks are limited by simple structure and composition. The decellularized matrix has been tried to amplify bioactivity of bioink. Herein, we modify the conventional protocol and get a novel hydrogel matrix from the discarded human tissues. The matrix could be used as bioink in 3D printing and one tissue derived decellularized extracellular matrix was named dECM-X (Figure 1A). Excitingly, it showed that the printed structures from dECM-X supported stem cell proliferation and in the animals there would be less immune-rejection compared to dECM from some other tissues. Based on the in vivo study of the printed scaffolds, it is demonstrated that tissue injuries such as skin burning/damage can be immediately repaired because of the high bioactivity of vascular network regeneration and tissue remodelling. Furthermore, for the decellularized work, we have generated some dECM patches from the uterus. After implantation, there would be myometrium and endometrium formation to recapitulate native tissue structure (Figure 1B). Importantly, there were also glands and blood vessels organization and the isolated tissues could react to estrogen and progestin. Finally, the host rats could be pregnant and healthy offsprings were generated. The further work will concentrate on the composition analysis. Summarily, the ECM from natural tissues provides many opportunities for bioink development and tissue reestablishment. Our work also promotes the application of ECM biomaterials.