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Microbial extracellular hydrolase activity and kinetics in the deep ocean
编号：1379 访问权限：仅限参会人 更新：2026-09-01 00:09:25 浏览：0次 张贴报告

报告开始：暂无开始时间（Asia/Shanghai）

报告时间：暂无持续时间

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摘要
The deep-sea carbon cycle is a critical yet still enigmatic component of Earth’s climate system, where high hydrostatic pressure severely limits our ability to probe in situ microbial-driven processes and estimate carbon fluxes. A key bottleneck lies in characterizing specific extracellular hydrolase activities under in situ pressure regimes, as these hydrolases mediate the rate-limiting step of organic matter degradation. We developed a pressure-retaining sampling and incubation system to measure potential activity and apparent kinetics of extracellular hydrolases secreted by deep-sea microbial communities in the Western Pacific under simulated in situ pressure. Our results reveal a pressure-dependent divergence in the specific extracellular hydrolase responses: increasing hydrostatic pressure (with depth) consistently suppressed bulk alkaline phosphatase activity, while markedly stimulating β-glucosidase, β-N-acetylglucosaminidase, and leucine aminopeptidase activities. Community-level enzyme kinetic analyses revealed increased substrate affinity (reflected by decreased half-saturation constants, Km) of β-glucosidase, β-N-acetylglucosaminidase, and alkaline phosphatase under high hydrostatic pressure, whereas leucine aminopeptidase showed decreased affinity but the most pronounced pressure-induced increase in maximum reaction rate. Based on these observed extracellular hydrolase activities, an ecoenzymatic stoichiometry model predicted elevated microbial growth efficiency under high hydrostatic pressure compared to atmospheric pressure, with a concomitant relief of carbon limitation, as validated by concurrent measurements of biomass production and respiration under simulated high pressure. This study demonstrates that despite low organic matter concentrations, the deep ocean sustains microbial communities with high carbon-use efficiency, driving an enhanced proportion of carbon retention in biomass and thereby promoting carbon storage in the ocean interior.
 
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报告人
Yizu Zhu
State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University

稿件作者
Yizu Zhu State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University
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重要日期

  • 会议日期

    01月12日

    2027

    至

    01月15日

    2027

  • 07月21日 2026

    初稿截稿日期

  • 01月15日 2027

    注册截止日期

主办单位
State Key Laboratory of Marine Environmental Science, Xiamen University (MEL)
Department of Earth Sciences, National Natural Science Foundation of China (NSFC)
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