56 / 2024-10-16 11:24:25

Myogenic Enhancer Snatching Promotes Adipogenic Differentiation during Chromatin Accessibility Reprogramming Mediated by Lineage-Specific Transcription Factors

Trans-differentiation，Hi-C，Enhancer snatch，Chromatin reprogramming

The myoblast-to-adipocyte trans-differentiation model is crucial for studying the regulatory mechanisms underlying ectopic fat deposition and cell fate determination. However, the comprehensive dynamics of chromatin accessibility reprogramming and chromatin interactions during this process remain largely unexplored. To address this, we performed an integrative analysis of ATAC-seq, RNA-seq, and Hi-C data on a cocktail-induced adipogenesis model in C2C12 myoblasts. Our findings revealed significant reprogramming of chromatin accessibility driven by adipogenic lineage-specific transcription factors (TFs), particularly Cebps and Stats, during trans-differentiation. Notably, 63.46% of the distal open chromatin regions (OCRs) with increased accessibility were shared between myogenesis and adipogenesis. Further joint analysis with Hi-C data suggests that these pre-established enhancers in myoblasts undergo a "regulatory redirection," shifting their influence from myogenic to adipogenic genes—a phenomenon we termed "enhancer snatch." This model was validated by knocking out a key "snatched" enhancer (Enhancer-R/L), which was found to be a critical regulator of both Rbl1 and Lbp expression. In summary, our study uncovers a mechanism by which pre-established enhancers in the original cell lineage are predominantly utilized by lineage-specific TFs during chromatin reprogramming, thereby altering the cell's differentiation trajectory.