366 / 2024-02-29 22:03:24

Heterotrophic Nitrification-Aerobic Denitrification and Microbial Community Shift in BAF Bioaugmented with Strains ID2+ID5+ID8 for Rural Domestic Sewage

Heterotrophic nitrification-aerobic denitrification, Compound bacterial agent, Rural nitrogenous sewage

This study focuses on addressing nitrogen pollution in rural domestic sewage by investigating the bioenhanced denitrification effect of a new heterotrophic nitrification-aerobic denitrification compound bacterial agent (composed of Pseudomonas sp. ID2, ID5, and ID8) and microbial response mechanisms. The compound bacterial agent was added to a biological aerated filter (BAF) to analyze its impact on nitrogen removal performance. High-throughput sequencing technology was used to monitor changes in the bacterial community structure in BAF during the bioaugmentation process. The results indicated that the average removal rates of NH₄⁺-N and TN increased by 15.52% and 23.49%, respectively. Additionally, no accumulation of NO₂^--N was observed during the initial stage of complex bactericide addition. As the reactor continued to operate, the removal rate of NH₄⁺-N in the bactericide enhanced group was 12.15% and 3.1% higher than that in the blank control group during the second and third stages, respectively. Microbial community analysis revealed a shift in dominant bacterial genera towards Hyphomicrobium, Candidatus Asysphera, and Nitrospira, with an increase in Klebsiella abundance, showcasing the adaptability of the composite inoculant. Overall, the study confirmed the potential of the heterotrophic nitrification-aerobic denitrification compound bacterial agent in enhancing nitrogen removal efficiency in rural domestic sewage and provided valuable microbiological insights for optimizing biological treatment systems.