Amidst the rapid advancement of electric vehicles and energy storage systems, the thermal safety issue of module has emerged as a critical concern and a focal point for the industry. Although the TR behavior of batteries has garnered significant attention, the sensitivity to prestress during TR events remains underexplored. This study highlights the impact of pre-stress on TRP through experimental research, revealing that increased prestress shortens the duration of TRP, intensifies the TR reactions, worsens temperature consistency (TC), and escalates the severity of TR hazards. However, under the prestress exceeds 204kg, a long-term and violent combustion reaction within the battery consume flammable gases, reducing gas release and mitigating the gas hazards for TR. In addition, in order to quantify the effect of prestress on TRP, a method combining spearman correlation analysis and entropy weight was employed, indicating that prestress has a significant impact on thermal accumulation, with the sensitivity is 0.236, but has the least impact on the TRP duration, with the sensitivity is 0.043. In addition, in the module, 2# is the most sensitive to prestress with the sensitivity is 0.102, while 1# and 3# are 0.074 and 0.092. These findings are of significant theoretical and practical importance for guiding the optimization of module design and enhancing their thermal stability and safety under extreme conditions.