104 / 2023-07-18 15:47:41

Mechanisms and effects of novel ammonifying microorganisms on nitrogen ammonification in cow manure waste composting

Environmental protection; Ammonification; Ammonifying microorganisms; Nitrogen conversion; Cow manure; Compost

It is essential to reduce nitrogen losses and to improve nitrogen conversion during organic waste composting because of environmental protection and sustainable development. To reveal newly domesticated ammonifying microorganisms (AM) cultures on the ammonification and nitrogen conversion during the composting, the screened microbial agents were inoculated at 5% concentration (in weight basis) into cow manure compost under five different treatments: sterilized distilled water (Control), Amm-1 (mesophilic fungus-F1), Amm-2 (mesophilic bacterium-Z1), Amm-3 (thermotolerant bacterium-Z2), and Amm-4 (consortium: F1, Z1, and Z2), and composted for 42 days. Compared to control, AM inoculation prolonged the thermophilic phases to 9–19 days, increased the content of NH₄⁺-N to 1.60–1.96 g/kg in the thermophilic phase, reduced N₂O and NH₃ emissions by 22.85%–61.13% and 8.45%–23.29%, increased total Kjeldahl nitrogen, and improved cell count and viability by 12.09%–71.33% and 66.71%–72.91%. AM was significantly associated with different nitrogen and microbial compositions. The structural equation model (SEM) reveals NH₄⁺-N is the preferable nitrogen for the majority of bacterial and fungal growth and that AM is closely associated with the conversion between NH₃ and NH₄⁺-N. Among the treatments, inoculation with Amm-4 was more effective, as it significantly enhanced the driving effect of the critical microbial composition on nitrogen conversion and accelerated nitrogen ammonification and sequestration. This study provided new concepts for the dynamics of microbial in the ammonification process of new AM bacterial agents in cow manure compost, and an understanding of the ecological mechanism underlying the ammonification process and its contribution to nitrogen (N) cycling from the perspective of microbial communities.