4 / 2021-09-19 17:31:48

Phophorphobide A, a chlorophyllin derivative extracted from Gracilaria lemaneiformis, for photodynamic therapy of human cancer

Phophorphobide A Photodynamic therapy human cancer

Abstract:  Objective Phophorphobide-a (Pheo), a second generation Photosensitizer, is a kind of anionic porphyrin derivative, which is the decomposition product of chlorophyll-A in plant cells. In this study, the phototoxicity and darktoxicity of Pheo on breast cancer cells (McF-7) were investigated by MTT assay. Method cells were seeded (8 × 10³ cells per well) into 96-well plates in culture medium and incubated at 37℃ for 24 h. Then, cells were treated with predetermined concentrations（10μm/L, 15μm/L, 2.5μm/L, 1.25μm/L, 0.625μm/L, 0.3125μm/L）of Pheo for 4 h. Next, cells were rirradiated with a 670-680 nm laser (10 min). After culturing for another 24 h, the viability of the final irradiated cells was determined via the MTT assay. Results Pheo revealed low darktoxicity on cancer cells at all tested concentrations and high photoxicity after laser irradiation on the cancer cells at concentrations more than 2.5μm/L. Conclusion Pheo has significant phototoxicity to breast cancer cells (McF-7). Pheo, with low darktoxicity and high photoxicity, is an excellent photosensitizer. It has great potential ability in photodynamic therapy.