26 / 2021-10-09 11:14:58

Two new cerebroside metabolites from the marine fungus Hortaea werneckii

cerebroside, RAW264.7

Two new cerebroside metabolites were isolated and purified from the fermented extract of Hortaea werneckii in the marine fungus, namely Hortacerebroside A(1): 1-

O-β-D-glucopyranosyl-(2S,3R,4E,8E)-2-[(2′R)-2′-hydroxy-3′-Pentadecenoyl]-9-met-hyl-heneicosa-4,8-dien-1,3-diol and Hortacerebroside B(2): 1-O-β-D-glucopyranosyl-

(2S,3R,4E,8E)-2-[(2′R)-2′-hydroxypentadecanoylamino]-9-methyl-heneicosa-4,8-dien-1,3-diol were elucidated by spectroscopic analysis and by comparison of the spectral data with those of related cerebroside analogues. In addition, the two compounds have a significant inhibitory effect on NO produced by lipopolysaccharide  (LPS) stimulated RAW264.7 macrophages. The IC₅₀ value of Hortacerebroside A (1) was 6.69 µM, and the IC₅₀ value of Hortacerebroside B (2) was 5.49 µM.