Myocardial infarction (MI) is one of the leading causes of global mortality. The dysfunction of cardiac muscles leads to heart failure, which requires the heart transplantation at the end-stage of MI. However, the number of donors cannot meet this demand. Tissue engineering has shown possibility of fabricating tissues or organs in vitro. However, it is still challenging to fabricate cardiac muscles that consist of highly aligned cardiomyocytes (CMs). These highly aligned CMs largely contribute to their contraction, maturation, and the anisotropic electrical and force propagation. In this study, we fabricate micropatterned cardiac patches, where human embryonic stem cell-derived cardiomyocytes (hESC-CMs) are seeded on. Immunofluorescence staining of nuclei and F-actin demonstrated the guided alignment of hESC-CMs.