42 / 2019-06-23 21:31:49

A bilayered GelMA/PEGDA-based nerve guidance with seeded BMSC for peripheral nerve regeneration

Gelatin methacryloyl(GelMA),nerve guide,PEGDA,3D printing

全体主题 > Engineering tissues and organs

Rupture of a nerve is a debilitating injury and is usually presented with poor quality of life in patients. The gold standard of repair is the use of an autologous graft. Autografts however lead to inadequate functional recovery and involve risks due to secondary surgery at the donor site. Here we report a novel 3D-printed bilayered gelatin methacrylated/Poly (ethylene glycol) diacrylate (GelMA/PEGDA)-based conduit combined with bone marrow stem cells (BMSCs). This study examined the physical, mechanical and biological properties of the conduits for neural reconstruction applications. In vitro characterization demonstrated that the conduit possessed strong mechanical strength with Young’s modulus of 3.23MPa and Compressive modulus of 1.61N/mm. In vitro cell culture showed that PC12 cells attachment, spreading and proliferation on the GelMA/PEGDA seeded with BMSCs were great, with results close to that of on the petri dish. Suture pullout experiments revealed that the conduits were better able to resist suture pullout. Collectively, this study suggests that GelMA/PEGDA-based bilayered nerve conduit seeded with BMSC shoes great promise for improving the current state of art in peripheral nerve repair strategies.