摘要详情

ID / 提交时间

767 / 2019-06-15 01:08:15

标题

Genetic and Biochemical Investigation of photosynthesis as the Source of Reducing Power for Fatty Acid Biosynthesis in Arabidopsis Seeds

关键字

Reducing power,Fatty acid,Chlorophyll synthase mutant,Photosynthesis,Differentially expressed genes

主题及专题

全体主题 > Photosynthesis

状态

摘要待审

作者

Chinedu Nwafor / National Research Centre of Rapeseed Engineering and Technology and College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

Delin Li / National Research Centre of Rapeseed Engineering and Technology and College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

YongTia Yin / Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074 China

Maoteng Li / Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074 China

Liang Guo / National Key Laboratory of Crop genetic improvement, Huazhong Agricultural University

Edgar Cahoon / University of Nebraska Lincoln

Chunyu Zhang / National Research Centre of Rapeseed Engineering and Technology and College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

摘要

Increasing seed oil content is a major target for manipulation in genetic improvement of oil seed crops. However, a better understanding of the contribution of seed photosynthesis to overall oil biosynthesis in seeds is still lacking. In general, plant seeds are classified as either photoheterotrophic green seeds or heterotrophic non-green seeds depending on the color of seed during development. Green seeds are known to use chlorophyll to harvest light energy for photosynthesis. The consensus based on previous research is that fatty acid (FA) biosynthesis in green seeds is solely dependent on the light reaction of photosynthesis for generation of reductant (NADPH) and energy (ATP). but these studies lack genetic evidence, besides emerging data suggests Arabidopsis seeds deficient in light-induced photosynthetic capacity accumulated FA comparable to the wild-type at maturity. This study provides the framework for the genetic investigation of sources of reducing power required for de novo FA biosynthesis in seeds. By exploring the chlorophyll synthase knockout mutant and its complement linked with DsRed-fluorescent protein we reveal the contribution of seed photosynthesis to FA accumulation in Arabidopsis seeds.